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rabbit polyclonal antibody directed against collagen i  (Biotrend Chemicals)

 
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    Biotrend Chemicals rabbit polyclonal antibody directed against collagen i
    Rabbit Polyclonal Antibody Directed Against Collagen I, supplied by Biotrend Chemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+antibody+directed+against+collagen+i/rabbit+antibody+against+collagen+i/pm39637045-60-24-26
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal antibody directed against collagen i - by Bioz Stars, 2026-09
    90/100 stars

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    Incubation:

    Article Title: Protease-activated receptor 2 deficient mice develop less angiotensin II induced left ventricular hypertrophy but more cardiac fibrosis
    Article Snippet: For immunohistochemistry endogenous peroxidase was blocked by incubation with 3% H 2 O 2 for 20 min followed by incubation in blocking solution (1% bovine serum albumin (BSA) in Tris(hydroxymethyl)aminomethan-buffered saline supplemented with 0.05% Tween 20 (all purchased from Sigma Aldrich, Deisenhofen, Germany)) for 10 min at room temperature to prevent unspecific binding. .. The sections were incubated with the following primary antibodies were diluted in blocking solution at 4°C overnight: a rabbit polyclonal antibody directed against collagen I (Biotrend Chemikalien GmbH, Köln, Germany); a goat polyclonal antibody directed against human collagen IV (Southern Biotech, Birmingham, AL, USA), a mouse monoclonal antibody directed against alpha smooth muscle actin (SMA) (DAKO, Glostrup, Denmark), a rat monoclonal antibody directed against CD31(DAKO, Glostrup, Denmark); a goat polyclonal antibody directed against P-Smad 2/3 (sc-11769; Santa Cruz Biotechnology, Dallas, TX, USA); a rabbit polyclonal antibody directed against p44/42 MAPK (ERK1/2; Thr202/Tyr204) (Cell Signaling Technology Europe B.V., Leiden NL). .. After washing with Tris-buffer pH 7.6 supplemented with 0.05% Tween 20, sections were incubated for 30 minutes at room temperature with appropriate biotinylated secondary antibodies diluted 1:500 in blocking solution: a horse anti mouse IgG (BA-2001); a rabbit anti-goat IgG (BA-5000); a goat anti-rabbit IgG (BA-1000) (all purchased from Vector laboratories).

    Article Title: Protease-activated receptor 2 deficient mice develop less angiotensin II induced left ventricular hypertrophy but more cardiac fibrosis.
    Article Snippet: For immunohistochemistry endogenous peroxidase was blocked by incubation with 3% H2O2 for 20 min followed by incubation in blocking solution (1% bovine serum albumin (BSA) in Tris(hydroxymethyl)aminomethanbuffered saline supplemented with 0.05% Tween 20 (all purchased from Sigma Aldrich, Deisenhofen, Germany)) for 10 min at room temperature to prevent unspecific binding. .. The sections were incubated with the following primary antibodies were diluted in blocking solution at 4 ̊C overnight: a rabbit polyclonal antibody directed against collagen I (Biotrend Chemikalien GmbH, Köln, Germany); a goat polyclonal antibody directed against human collagen IV (Southern Biotech, Birmingham, AL, USA), a mouse monoclonal antibody directed against PLOS ONE | https://doi.org/10.1371/journal.pone.0310095 December 5, 2024 3 / 22 alpha smooth muscle actin (SMA) (DAKO, Glostrup, Denmark), a rat monoclonal antibody directed against CD31(DAKO, Glostrup, Denmark); a goat polyclonal antibody directed against P-Smad 2/3 (sc-11769; Santa Cruz Biotechnology, Dallas, TX, USA); a rabbit polyclonal antibody directed against p44/42 MAPK (ERK1/2; Thr202/Tyr204) (Cell Signaling Technology Europe B.V., Leiden NL). .. After washing with Tris-buffer pH 7.6 supplemented with 0.05% Tween 20, sections were incubated for 30 minutes at room temperature with appropriate biotinylated secondary antibodies diluted 1:500 in blocking solution: a horse anti mouse IgG (BA2001); a rabbit anti-goat IgG (BA-5000); a goat anti-rabbit IgG (BA-1000) (all purchased from Vector laboratories).

    Blocking Assay:

    Article Title: Protease-activated receptor 2 deficient mice develop less angiotensin II induced left ventricular hypertrophy but more cardiac fibrosis
    Article Snippet: For immunohistochemistry endogenous peroxidase was blocked by incubation with 3% H 2 O 2 for 20 min followed by incubation in blocking solution (1% bovine serum albumin (BSA) in Tris(hydroxymethyl)aminomethan-buffered saline supplemented with 0.05% Tween 20 (all purchased from Sigma Aldrich, Deisenhofen, Germany)) for 10 min at room temperature to prevent unspecific binding. .. The sections were incubated with the following primary antibodies were diluted in blocking solution at 4°C overnight: a rabbit polyclonal antibody directed against collagen I (Biotrend Chemikalien GmbH, Köln, Germany); a goat polyclonal antibody directed against human collagen IV (Southern Biotech, Birmingham, AL, USA), a mouse monoclonal antibody directed against alpha smooth muscle actin (SMA) (DAKO, Glostrup, Denmark), a rat monoclonal antibody directed against CD31(DAKO, Glostrup, Denmark); a goat polyclonal antibody directed against P-Smad 2/3 (sc-11769; Santa Cruz Biotechnology, Dallas, TX, USA); a rabbit polyclonal antibody directed against p44/42 MAPK (ERK1/2; Thr202/Tyr204) (Cell Signaling Technology Europe B.V., Leiden NL). .. After washing with Tris-buffer pH 7.6 supplemented with 0.05% Tween 20, sections were incubated for 30 minutes at room temperature with appropriate biotinylated secondary antibodies diluted 1:500 in blocking solution: a horse anti mouse IgG (BA-2001); a rabbit anti-goat IgG (BA-5000); a goat anti-rabbit IgG (BA-1000) (all purchased from Vector laboratories).

    Article Title: Protease-activated receptor 2 deficient mice develop less angiotensin II induced left ventricular hypertrophy but more cardiac fibrosis.
    Article Snippet: For immunohistochemistry endogenous peroxidase was blocked by incubation with 3% H2O2 for 20 min followed by incubation in blocking solution (1% bovine serum albumin (BSA) in Tris(hydroxymethyl)aminomethanbuffered saline supplemented with 0.05% Tween 20 (all purchased from Sigma Aldrich, Deisenhofen, Germany)) for 10 min at room temperature to prevent unspecific binding. .. The sections were incubated with the following primary antibodies were diluted in blocking solution at 4 ̊C overnight: a rabbit polyclonal antibody directed against collagen I (Biotrend Chemikalien GmbH, Köln, Germany); a goat polyclonal antibody directed against human collagen IV (Southern Biotech, Birmingham, AL, USA), a mouse monoclonal antibody directed against PLOS ONE | https://doi.org/10.1371/journal.pone.0310095 December 5, 2024 3 / 22 alpha smooth muscle actin (SMA) (DAKO, Glostrup, Denmark), a rat monoclonal antibody directed against CD31(DAKO, Glostrup, Denmark); a goat polyclonal antibody directed against P-Smad 2/3 (sc-11769; Santa Cruz Biotechnology, Dallas, TX, USA); a rabbit polyclonal antibody directed against p44/42 MAPK (ERK1/2; Thr202/Tyr204) (Cell Signaling Technology Europe B.V., Leiden NL). .. After washing with Tris-buffer pH 7.6 supplemented with 0.05% Tween 20, sections were incubated for 30 minutes at room temperature with appropriate biotinylated secondary antibodies diluted 1:500 in blocking solution: a horse anti mouse IgG (BA2001); a rabbit anti-goat IgG (BA-5000); a goat anti-rabbit IgG (BA-1000) (all purchased from Vector laboratories).



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